CHM 222LW - Organic Biochemistry Laboratory

1. Communicate effectively.
   1. Demonstrate understanding by reading, speaking, and writing.
   2. Employ critical writing and active listening to obtain or convey information.
   3. Collect and analyze data.
   4. Identify trends, solve problems, and conclude logically by integrating concepts.
2. Demonstrate an understanding of the importance of laboratory safety and maintenance.
   1. Complete the agreement provided after reading the safety protocols and regulations of the science laboratory.
   2. Conduct safe lab procedures and recognize common laboratory hazards.
   3. Identify and locate all safety features within the laboratory (eye wash fountains, fire extinguishers, fire alarms fire blankets, etc.).
   4. Complete the agreement provided after reading the procedures of safe and ethical use of equipment and instrumentation used in the laboratory.
   5. Dispose of all waste materials properly (chemicals, sharps, broken glassware), if necessary review Safety Data Sheets.
   6. Use personal protection (e.g. goggles, gloves, lab coat) properly.
   7. Clean up all work areas and return all equipment to designated areas before leaving the laboratory.
   8. Report any accidents, incidents or breakages immediately.
3. Record experimental work to promote learning of good laboratory practices
   1. Locate Safety Data Sheets as needed.
   2. Use a laboratory notebook to communicate experimental concepts and results.
   3. Record and analyze the results and data of an experiment.
   4. Produce reports to inform others of your progress in the laboratory.
4. Apply chemical methods to biochemical systems
   1. Use basic chemistry skills to make reagents in a laboratory setting including the concept of moles and molar solutions, percent solutions, normality, and dilutions.
   2. Perform and demonstrate an understanding of the principles of extraction, chromatography, serial dilutions, buffers and pH.
   3. Use chemical methods to explain colorimetric assays, enzyme catalysis and protein concentrations.
   4. Use chemical tests and indicators to determine the presence of specific organic functional groups found in biological macromolecules.
   5. Use various tests and reactivity’s to compare and identify specific functional groups found in biochemical systems.
5. Demonstrate the competent use of laboratory instrumentation and equipment
   1. Accurately use critical pieces of equipment like micropipetors, balances, spectrophotometers, hot plates and water baths etc.
   2. Exhibit proper handling of enzymes, solutions, solids and glassware used in biochemical experiments and analysis.
   3. Design and conduct experiments with proper safety features.
   4. Conduct controlled experiments without wasting reagents and time.
   5. Explain the use of UV-visible spectrometers, IR spectrometers for quantitative and qualitative analysis.
   6. Use structural drawing programs to draw major macromolecules.
6. Demonstrate use of equipment to study enzyme kinetics
   1. Explain how enzymes are specific and selective catalysts with reference to the chemical properties of amino acids and prosthetic groups.
   2. Define activation energy and describe how it is related to reaction rates, free energy and bonding energy.
   3. Conduct enzyme kinetics to calculate Km and Vmax.
   4. Conduct enzyme kinetics to identify competitive, non-competitive and uncompetitive inhibitors using Lineweaver-Burke plots.
   5. Explain the reactivity of enzymes with variables such as pH, substrate concentration, temperature and enzyme concentration.